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data-config="" data-state="true"> <div id="1nimltqo" class="appMask"></div> <div id="1nimltqo" class="appContent"> <div id="1nimltqo" class="datum_info"> <h1 class="title">選定ELISA類型確定向?qū)?/h1> <div id="1nimltqo" class="bgcolor_01"> <div id="1nimltqo" class="bgcolor_sum"> <div id="1nimltqo" class="left">日期：2024-11-21 21:06</div> <div id="1nimltqo" class="right">瀏覽次數(shù)：163</div> </div> <div id="1nimltqo" class="bgcolor_sum01"> 摘要： ELISA技術(shù)可用于測(cè)定抗原，也可用于測(cè)定抗體。 ELISA方法中有三個(gè)必要的試劑： (1) 固相的抗原或抗體，即"免yi吸附劑"（immunosorbent）； (2) 酶標(biāo)記的抗原或抗體，稱為"結(jié)合物"（conjugate）； (3) 酶反應(yīng)的底物。一般可將ELISA分為以下幾種類型，實(shí)驗(yàn)先需要根據(jù)自己的待檢物來確定試劑盒類型。 1) 雙抗體夾心法：針對(duì)抗原分子上兩個(gè)不同抗原決定簇的單克隆抗體分別作為固相捕獲抗體和酶標(biāo)檢測(cè)抗體。適用于測(cè)定二價(jià)... </div> </div> <div id="1nimltqo" class="content"> <p> ELISA技術(shù)可用于測(cè)定抗原，也可用于測(cè)定抗體。 </p> <p> <br> </p> <p> ELISA方法中有三個(gè)必要的試劑： </p> <p> <br> </p> <p> (1) 固相的抗原或抗體，即"免yi吸附劑"（immunosorbent）； </p> <p> <br> </p> <p> (2) 酶標(biāo)記的抗原或抗體，稱為"結(jié)合物"（conjugate）； </p> <p> <br> </p> <p> (3) 酶反應(yīng)的底物。 </p> <p> <br> </p> <p> 一般可將ELISA分為以下幾種類型，實(shí)驗(yàn)先需要根據(jù)自己的待檢物來確定試劑盒類型。 </p> <p> <br> </p> <p> 1) 雙抗體夾心法：針對(duì)抗原分子上兩個(gè)不同抗原決定簇的單克隆抗體分別作為固相捕獲抗體和酶標(biāo)檢測(cè)抗體。適用于測(cè)定二價(jià)或二價(jià)以上的大分子抗原如血漿中的細(xì)胞因子，不適用于測(cè)定半抗原及小分子單價(jià)抗原。同理，也有雙抗原夾心法測(cè)抗體。 </p> <p> <br> </p> <p> 2) 直接法：將抗原直接固定在固相載體上，加入酶標(biāo)記的一抗，即可測(cè)定抗原總量。此法操作手續(xù)簡(jiǎn)短，因無須使用二抗可避免交互反應(yīng)。 </p> <p> <br> </p> <p> 3) 間接法：間接法是檢測(cè)抗體常用的方法，其原理為利用酶標(biāo)記的抗抗體（抗人免yi 球蛋白抗體）來檢測(cè)與固相抗原結(jié)合的待檢抗體。 </p> <p> <br> </p> <p> 4) 競(jìng)爭(zhēng)法：小分子抗原或半抗原因缺乏可作夾心法的兩個(gè)以上的位點(diǎn)，因此不能用雙抗體夾心法進(jìn)行測(cè)定，可以采用競(jìng)爭(zhēng)法模式。當(dāng)抗原材料中的干擾物質(zhì)不易除去，或不易得到足夠的純化抗原時(shí)，可用此法檢測(cè)特異性抗體。 </p> </div> <div id="1nimltqo" class="datumrelate"> <span>下一篇：</span> <a href="/banjbio_Affiche_11756211.html" title="PCR技術(shù)必須3次循環(huán)得到目的基因分析">PCR技術(shù)必須3次循環(huán)得到目的基因分析</a> <br> <span>上一篇：</span> <a 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